Hardware Manual3 min read

Treadmill Air Puff Manual

This manual covers the components, assembly, and software configuration of the Treadmill Air Puff system, which delivers air stimuli to animals on a treadmill through an air compressor, solenoid box, and tubing network controlled by a software interface.

Updated Feb 7, 2026
3 sections

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Treadmill Air Puff Manual

Air Puff Components

  • 32-ft long air tubing (blue hose) between the air compressor and the solenoid box
  • Solenoid box
  • 5 12-ft long clear air tubing between the solenoid box and treadmill
  • 1 cable between the solenoid box and the control box

Air Puff Component Connections

Connection of sensors installed in covers to the treadmill

Air tubing connections between treadmill and solenoid box

Air input to solenoid box

Blue air hose between solenoid box and air compressor (tube 8 mm OD and 5 mm ID)

Connection between solenoid box and control box

Connection to air compressor

One 32 feet tubing and tube fitting are provided. If you need additional tube fitting, it can be found on Amazon.com (8mm or 5/16 inch OD 5mm ID):

https://www.amazon.com/gp/product/B07RM5WWBT/ref=ppx_yo_dt_b_asin_title_o02_s00?ie=UTF8&psc=1

Two pieces of steel plug are provided. If you need additional pieces, they can be found in Home Depot or online.

Air compressor

The air compressor (no more than 8kg/cm2 or 110 psi) is required.

The air compressor can be purchased in Home Depot or online. Some labs provide central air. Below is an example model our clients are using:

https://www.amazon.com/Silentaire-Silent-Running-Airbrush-Compressor/dp/B00O318SLA

Connector for this air compressor:

We use a push lock style 8mm tube x ¼ inch standard connector with that compressor. We unscrew and remove the output fitting that comes with the air compressor and install the push lock connector in its place. This fitting can be found here:

https://www.aignepusa.com/products/alpha-swift-fit-universal-fittings/alpha-swift-fit-composite-body-fittings/straight-male?id=25

Software Setting

In order for air puff or shock to work, the covers have to be put in place because the sensors inside the covers are required to detect the animal moves.

Shock level is typically set at 1.4-1.7mA for mouse and 1.7-2.2mA for rat.

Control Screen Buttons

Gas – When the button is pressed, the air stimulus is enabled. When the button is released, the air stimulus is disabled.

Gas_T – If the value is none, the air puff will continue to work every time the animal is in the grid area. If the value is set to a number, say 5, the air puff will be delivered 5 times.

Note that the air puff and shock can work at the same time if both of them are enabled.

Shock/air puff occurs when it is enabled and the animal falls in the grid areas (detected by sensors).

Shock/air puff indicator light blinks when the shock occurs.

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